An improved ternary vector system for Agrobacterium-mediated rapid maize transformation
نویسندگان
چکیده
منابع مشابه
Agrobacterium Mediated Transformation of Maize (Zea mays L.)
Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
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Wheat is the world’s largest volume crop traded internationally. Foliar diseases are the main biotic restriction reducing yield in wheat crops. Wheat productivity can be enhanced through controlling foliar disease through the use of resistant cultivars. Plant breeders are providing considerable attention to wheat genetic improvement from the past few years to minimize losses due to pests and pa...
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We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciens standard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L-1 L-cysteine. Inclusion of L-cysteine in cocultivation medium lead to an i...
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We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciens standard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L 1 l-cysteine. Inclusion of l-cysteine in cocultivation medium lead to an i...
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The plasmid vector pGreenII is widely used to produce plant transformants via a process that involves propagation in Escherichia coli However, we show here that pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death. In addition, we describe a new version of pGreenII that does not cause these effects, thereby removing the s...
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ژورنال
عنوان ژورنال: Plant Molecular Biology
سال: 2018
ISSN: 0167-4412,1573-5028
DOI: 10.1007/s11103-018-0732-y